A Further Study of Human Seminal Plasma Lactate Dehydrogenase-c Cldh-c4): Kinetic Properties of Ldh-c4
نویسنده
چکیده
Lactate dehydrogenase.C. (LDH-C.) from human seminal plasma was purified by 40~60% (NH.)2S0. precipitation, Oxamate-Sepharose 4B affinity column chromatography and AMP affinity column chromatography. Enzyme kinetic studies of human seminal plasma LDH-C, were performed with purified LDH-C.. The human LDH-C. exhibits a much higher affinity for pyruvate and a-ketovalerate than lactate. Apparent Km values for pyruvate, a-ketovalerate and lactate were 7. M and 6.7 x 10-3 M, respectively. The human seminal plasma LDH-C, was severely inhibited by increasing concentration of substrate, especially lactate. These kinetic properties were compared with those of previous reports of human spermatozoa.
منابع مشابه
A further study of human seminal plasma lactate dehydrogenase-C4 (LDH-C4): kinetic properties of LDH-C4.
Lactate dehydrogenase-C4 (LDH-C4) from human seminal plasma was purified by 40-60% (NH4)2SO4 precipitation, Oxamate-Sepharose 4B affinity column chromatography and AMP affinity column chromatography. Enzyme kinetic studies of human seminal plasma LDH-C4 were performed with purified LDH -C4. The human LDH-C4 exhibits a much higher affinity for pyruvate and alpha-ketovalerate than lactate. Appare...
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